Effect of cutting tool with micro-pits texture on wood cutting performance.

A reasonable micro-pits texture has been initially proved that it can improve friction characteristics between wood and cemented carbide surface and reduce surface friction coefficient. In order to study the cutting performance of the micro-texture when it is applied to the cutting tool for cutting wood more effectively, this paper selected micro-pit texture for studying influence of surface micro-texture cutting tool on wood cutting performance and cutting temperature, finding that when micro-pit cemented carbide cutting tool is adopted for turning the northeast China ash (Fraxinus spp.), it can reduce cutting force of turning and surface friction coefficient between rake face and cuttings. Moreover, for type A and type B cutting tools, when the texture parameters are that the diameter of the micro-pit is 80µm, the depth of the micro pit is: 10µm, area occupancy is 20% and the diameter of the micro-pit is 120µm, the depth of the micro-pit is 10µm and the area occupancy is 20%, the effect generated is the best. When a texture cutting tool is used for cutting, the decrease of the highest temperature in the cutting area is not very great, but the average temperature in the cutting area changes a lot, which is mainly caused by that micro-texture is processed at a position of the rake face close to the main cutting edge and that the highest temperature of cutting is mainly generated on the contact point between tool tip and wood. A reasonable micro-texture parameter can form a layer of liquid lubricating film on the up and down contact surfaces such that the direct contact between cemented carbide and northeast China ash is changed into indirect contact between lubricating films formed by the liquid so as to reduce the surface friction coefficient.

Morphological imaging and quantification of axial xylem tissue in Fraxinus excelsior L. through X-ray micro-computed tomography.

The popularity of X-ray based imaging methods has continued to increase in research domains. In wood research, X-ray micro-computed tomography (XµCT) is useful for structural studies examining the three-dimensional and complex xylem tissue of trees qualitatively and quantitatively. In this study, XµCT made it possible to visualize and quantify the spatial xylem organization of the angiosperm species Fraxinus excelsior L. on the microscopic level. Through image analysis, it was possible to determine morphological characteristics of the cellular axial tissue (vessel elements, fibers, and axial parenchyma cells) three-dimensionally. X-ray imaging at high resolutions provides very distinct visual insight into the xylem structure. Numerical analyses performed through semi-automatic procedures made it possible to quickly quantify cell characteristics (length, diameter, and volume of cells). Use of various spatial resolutions (0.87-5 µm) revealed boundaries users should be aware of. Nevertheless, our findings, both qualitative and quantitative, demonstrate XµCT to be a valuable tool for studying the spatial cell morphology of F. excelsior.

Apoplastic and symplastic phloem loading in Quercus robur and Fraxinus excelsior.

Whereas most of the research on phloem loading is performed on herbaceous plants, less is known about phloem loading strategies in trees. In this study, the phloem loading mechanisms of Quercus robur and Fraxinus excelsior were analysed. The following features were examined: the minor vein structure, the sugar concentrations in phloem sap by the laser-aphid-stylet technique, the distribution of photoassimilates in the mesophyll cells by non-aqueous fractionation, gradients of sugar concentrations and osmotic pressure, and the expression of sucrose transporters. The minor vein configurations of Q. robur and F. excelsior belong to the open type. Quercus robur contained companion cells in the minor veins whereas F. excelsior showed intermediary cells in addition to ordinary companion cells. The main carbon transport form in Q. robur was sucrose (~1M). In F. excelsior high amounts of raffinose and stachyose were also transported. However, in both tree species, the osmolality of phloem sap was higher than the osmolality of the mesophyll cells. The concentration gradients between phloem sap and the cytoplasm of mesophyll cells for sucrose were 16-fold and 14-fold for Q. robur and F. excelsior, respectively. Independent of the type of translocated sugars, sucrose transporter cDNAs were cloned from both species. The results indicate that phloem loading of sucrose and other metabolites must involve active loading steps in both tree species. Quercus robur seems to be an apoplastic phloem loader while F. excelsior shows indications of being a symplastic or mixed symplastic-apoplastic phloem loader.

Structural and physiological responses to ozone in Manna ash (Fraxinus ornus L.) leaves of seedlings and mature trees under controlled and ambient conditions.

Leaf-level microscopical symptom structure and physiological responses were investigated in seedlings experimentally exposed to ozone (O3) in indoor chambers (150 ppb, 8 h d(-1)/7 weeks), and field trees of Manna ash (Fraxinus ornus) exposed to ambient O3 (max 93 ppb/one growing season). Ozone-induced leaf injury, including leaf reddening and stippling, was observed in both seedlings and mature trees, but the morphology of injury in the stipples differed, being hypersensitive-like (HR-like) in the chamber seedlings and accelerated cell senescence (ACS) in the field trees. In both exposure conditions, the main structural impact of O3 was on the mesophyll and especially the upper assimilating cell layers. The main physiological impact was on carbon assimilation and on stomatal sluggishness. These effects were not due to stomatal structural injury and were more severe in juvenile compared to mature trees because of environmental (water availability, light) and constitutional (gas exchange capacity) factors and differences in the cell physiology processes (HR-like vs. ACS) triggered by ozone stress. Given the plasticity of plant responses to ozone stress, dose/response relationships for tree seedlings in the indoor chambers cannot be extrapolated to mature trees unless ambient conditions are closely simulated.